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Lately, novel radiation therapy methods using advanced laser technologies with ultra-high dose rates (“FLASH”), such as laser-accelerated particle beams and laser-driven ionizing radiation in pulses, with time spans from fs to ns started to emerge, having encouraging results in numerous studies concerning toxicity characterization [1,2]. Significant parameters of radiation therapies like high radiation intensities and short irradiation times are intended to improve treatment’s outcome and to have a better individualized treatment for each patients’ needs. One of the most important issues in radiotherapy is to find the right compromise between radiation’s efficacy to reduce the tumor and its toxicity. Also, it’s necessary to explain the mechanisms underlying the effects induced by ionizing radiation in biological matrices, and for that reason, endogenous molecules that provide information on toxicity are required. Amino acids can be selected as viable candidates for biomarkers due to their involvement in many cellular metabolic processes determined by high dose-rate radiations.
As forerunner experiments of laser driven irradiation trials, in this study we have investigated experimental models of gamma irradiated lyophilised glioblastoma cells lines. The evaluation of amino acids was performed both from a qualitative and quantitative point of view, using a Reversed Phase Liquid Chromatography with diode-array detection (RPLC–DAD) employing a precolumn derivatization method. This inquiry consisting in characterizing and profiling, allows us to have an insight into amino acids as biochemical evidences and their pathways in metabolic processes stimulated by radiation, in order to anticipate whether low toxicity using FLASH radiation was achieved.