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Negative pi-meson therapy has potential as an alternative radiotherapy method because it has a high radiation level, while healthy organs around it receive a low radiation dose. Negative pi-meson-based treatment is suitable for targeting tumours with many vital organs around them, one of which is prostate cancer. This study aims to analyse the dose and time of irradiation of negative pi-meson therapy for prostate cancer cases. The simulation is run on the PHITS program by modelling prostate cancer on the geometry of the body organs and tissues. The radiation source is negative pion with an intensity of 2.5 × 108 pion/second with an energy range of 40 MeV to 58 MeV. Optimization is done by placing the Bragg peak in the target depth area and determining the dose weight and irradiation time. The irradiation was carried out in 25 fractions with a dose per fraction is 2 Gy. The irradiation time per fraction was 91.23 seconds. The OARs reviewed in this study were the skin, bladder, testes, rectum & large intestine, spinal cord, kidney and soft tissue. The doses received by the OAR respectively were 0.09023 Gy; 1.35349 Gy; 0.22417 Gy; 0.38557 Gy; 0.00622 Gy; 0.00438 Gy and 0.11899 Gy.